Journal: Journal of Translational Medicine

Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

doi: 10.1186/1479-5876-9-198

Figure Lengend Snippet: Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® CellSTACK ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.

Article Snippet: As each brand of cell factory might have different surface coating properties that could affect DC differentiation and maturation, three commercially available cell factories were evaluated - 1) NunclonTMΔ Surface 1-tray Cell Factories; 2) Corning ® CellSTACK ® culture chambers; and 3) Corning ® cell culture ultra-low attachment CellSTACK ® chamber.

Techniques: Comparison, Cell Culture